Live cell culture monitoring and analysis anywhere and anytime

Live-cell imaging has become a desired analytical tool in many cell biology laboratories that operate in the field of neurobiology, developmental biology, pharmacology. Until now live-cell imaging has been difficult, because it required large costly high-end devices that are difficult to operate. The CytoSMART Lux2 provides a small, easy and affordable solution for live-cell imaging. The CytoSMART Lux2 is a compact inverted microscope for bright-field live cell imaging that makes live-cell imaging easy and affordable so it can be used by every biological laboratory. Even in routine cell culture processes.

No issues with environmental controls

Tight control of the environment (e.g. temperature, CO2) is one of the most critical factors determining the success or failure of a live-cell imaging experiment. Using a conventional microscope with a stage-top incubator it can be quite a challenge to maintain the cells in a healthy state and functioning normally for a longer period of time while being imaged. The CytoSMART Lux2 operates at low-voltage and is designed for safe use in a regular CO2 incubator.

Easy data storage and image analysis

The CytoSMART Lux2 can be set to record images at specific intervals for minutes, hours and days. In fact it is one of the few systems that can run for weeks. The recorded images are sent to the CytoSMART Connect Cloud where image analysis software analysis the proliferation by analyzing the occupied area (% confluence) of cell images over time. The image analysis data is graphically represented in a dashboard. Alerts can be set for confluency, meaning you will receive an automatic notification once your cell culture has reached a certain confluency and is ready for splitting or experiments, such as transfection.


With the CytoSMART Lux2 you have a big advantage over your colleagues and competitors.​ With our cloud based solution, you have access to the following application anywhere and anytime you need it:

  • Image cell division
  • Monitor cell growth and confluence
  • Analyze cell migration and wound healing or scratch assays
  • Study the behaviour of stem cells
  • Study chemotaxis

Easy access. Anywhere. Anytime.

Thanks to the cloud data storage and image analysis you can access your recording and view the cell culture in almost real-time from anywhere on any pc, tablet or mobile phone. All the recorded data such as images (.jpg files), time-lapse video (.avi files) or temperature data and confluency data (.csv files) can be downloaded for further processing.

Plates, Dishes, Flasks or microfluidic chips

A CytoSMART Lux2 can image cells in a wide range of cell culture vessels including slides, petri dishes, T-flasks, and microplates..

Growth is never by mere chance; it is the result of forces working together.



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How it works. Simple as 1-2-3

1. Place the CytoSMART Lux2 in the incubator. The combined power and data cable can be run either through a port in the back of the incubator or along the rubber sealing of the door. 

2. Connect the tablet to the device and the power cable to a power outlet.

3. Start the tablet. You’re set to go. You can now start recording a time-lapse of a cell culture.​​

Thanks to the cloud data storage and image analysis you can access your recording and view the cell culture in almost real-time from anywhere on any pc, tablet or mobile phone. All the recorded data such as images (.jpg files), time-lapse video (.avi files) or temperature data and confluency data (.csv files) can be downloaded for further processing.


Optics Bright-field only with digital phase contrast
Magnification 10x fixed objective
Fluorescence Filters N/A
Camera 5MP CMOS Sensor
Image Formats JPG
Image Size 1280 x 720 pixels
Field of View 2.4 x 1.5 mm
Video Rates Up to 8 frames/second (fps)
Culture Hold slides, 3 sizes Petri dishes, flask; custom available
Computer Requirements Windows 7, 8.1 or 10
Power Supply AC 100-240V, 2A, 10W, 50/60HZ
Dimensions 13.3 x 9.0 x 10.0 cm (L x W x H)
Weight 0.5 kg (1.1 lb)
Operating Conditions 0 - 42 °C, 5 - 95% RH non-condensing
Warranty 1 year parts & labor

Frequently Asked Questions

The CytoSMART Lux2 is a functional mini microscope that is designed to be placed inside an incubator to monitor cell cultures. Images are analyzed almost real time using image analysis software to provide insights into the cell confluency.

The dimensions of the mini microscope are 133mm (L) x 90 mm (W) x 100 mm (H) and weighs 0.5 kg. This corresponds to 5,2” (L) x 3,5” (W) x 3.9” (H) and a weight of 1.1 pound.
The included tablet is 25,7-cm (10,1-inch) which is 264 (L) x 173 (W) x 10 (H) mm and weighs 1.1 kg. This corresponds to 10.4” (L) x 6.8” (W) x 0.4” (H) and a weight of 2.2 pound.


The CytoSMART Lux2 is a brightfield only microscope. It has no filters for fluorescent dye detection.


The device is easy to clean using lint-free swipes and EtOH (80%) or IPA. Do not use Acetone to clean the device, also the device cannot be autoclaved.


No calibration by the user is needed for the device to operate.


After sterilizing with EtOH or IPA the device can be used in a cleanroom.


he device can be used with a Windows based tablet or PC. A WiFi or Ethernet internet connection is required to be able to connect to the CytoSMART server for data storage and analysis.

The CytoSMART Lux2 allows monitoring of a wide range of different culture dishes and flasks, such as:
  • ​T-flasks: T-25 up to T-250
  • Single well, multi-well plates (6, 12, 24, 96 well)
  • Flat tubes
  • Petri dishes
  • Slides
For larger flasks an adaptor plate may be required. This plate can be purchased at CytoSMART.

The CytoSMART Lux2 uses inverted bright-field microscopy. The sample is illuminated from the top and observed from below.


The CytoSMART Lux2 has a magnification equal to a microscope with a 10X and 20X lens.


The CytoSMART Lux2 records images at predefined intervals. At the start of a new project you can specify the interval rate between 1 - 60 minutes.


The device has a field of view of 2.5mm x 1.5 mm (3.75 mm2).


50 Gb of cloud storage is provided with the purchase of your CytoSMART Lux2, allowing for online storage and analysis of the image. This is enough for approximately 500.000 analyzed images.


Using a regular hemocytometer, the CytoSMART Lux2 can calculate the cell concentration. For more detailed cell concentration analysis we recommend using the Corning Cell Counter.

CytoSMART Technologies is a Dutch company whom wants to challenge every life science researcher to think differently and become the best in their field of expertise. We believe that high-end innovative tools should be accessible by all those trying to be at the forefront of scientific discovery. By working together, we can help those at the cutting edge of life science research. All to achieve greatness.

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