CytoSMART Lux3 FL

Automated fluorescence
imaging inside
your incubator

  • CytoSMART Lux3 FL

The CytoSMART Lux3 FL is a small fluorescence live-cell imaging microscope equipped with one brightfield and two fluorescent channels (green and red). The device enables researchers to unravel cellular processes in real-time, providing lots of valuable data about the experiments and answers if, when, how, and to what extend the cellular processes occur. CytoSMART’s first fluorescent cell imaging device allows users to track dynamic cellular processes with high specificity by taking high-quality images to create real-time time-lapse movies. Simultaneously, the cells are kept in a controlled environment inside a standard cell culture incubator.

Let Lux3 FL capture fluorescent and brightfield images for you

Even when you are not in the lab

Currently, fluorescent labeling is mostly used as an end-point measurement. However, time-lapse imaging of live cells can give much more information about biological processes. By using automated imaging at regular time intervals, the temporal resolution of the fluorescent data is increased, leading to even more relevant data about the cellular processes. In this way, with fluorescence microscopy, researchers can not only determine if a certain process has occurred, but also when it occurred and at what speed.

Live insight in cellular processes

Get the whole story and observe the development of cellular processes while they occur

The CytoSMART Lux3 fluorescence imaging microscope automatically creates time-lapse movies that contain many cellular features. The videos are made from inside the incubator without disturbing your cells and can be immediately accessed and analyzed remotely via the CytoSMART Cloud, providing real-time updates on your cell cultures and running experiments.

Applications of kinetic fluorescence assays

Using fluorescence live-cell imaging has become increasingly valuable in the fields of drug discovery, tissue engineering, immunology, immunotherapy, and cancer research. Various applications, such as monitoring cell viability, determining transfection efficiency, investigating co-cultures, and analyzing cellular processes, can benefit from the fluorescent labels and their detailed kinetic visualization. Many of these applications also benefit from the integrated image analysis included in the CytoSMART Cloud.

Analyze cells in their desired culture environment

Combine fuorescence and brighflield live-cell imaging inside the same incubator

Kinetic fluorescence imaging of cellular processes requires environmental control throughout the experiment. Because of its small size, the Lux3 FL fits inside any standard incubator. This enables you to perform fluorescent live-cell imaging experiments parallel to your regular cell cultures. Because of the remote data accessibility in the CytoSMART Cloud, your cells do not have to be disturbed during the experiment. This makes sure the cells are in the most optimal culture conditions as possible. Next to this, the remote data accessibility prevents you from making unnecessary lab visits.

Simultaneous analysis of multiple cell features

Expand the number of variables you can analyze in your cell culture

Using CytoSMART Lux3 FL, you can analyze green and red fluorescent tags simultaneously with your brightfield images. This not only saves you time but also provides tools to distinguish cells from each other or distinguish labeled structures within the cells. By tracking changes in the fluorescent signal over time, cell number changes or fluorescently tagged cellular components can easily be identified.

Technical specifications

Channels Brightfield, green and red fluorescence channels
Magnification 10X fixed objective - 20X digital zoom
Fluorescence filters Green: Excitation: 452/45 nm, Emission: 512/23 nm
Red: Excitation: 561/14 nm, Emission: 630/90 nm
Camera 6.4 MP CMOS
Data formats JPG, XLSX, MP4
Image size 2072 x 2072 pixels
Field of view 1.45 x 1.45 mm
Culture vessels Well-plates, petri dishes, flasks, microfluidic chips and custom culture vessels
Computer requirements Windows 10
Dimensions 166 x 140 x 135 mm (L x W x H)
Weight 1.3 kg
Operating conditions 5 - 40 °C, 20-95% humidity
Warranty 1-year parts and labor
Data storage Unlimited cloud storage
Research use only. Not intended for diagnostic purposes.

Videos

Frequently Asked Questions

The CytoSMART Lux3 FL is a small fluorescence live-cell imaging microscope equipped with one brightfield and two fluorescent channels (green and red). The device allows users to track dynamic cellular processes with high specificity by taking high-quality fluorescence images to create real-time time-lapse movies. Experiments' data can be accessed and analyzed remotely via the CytoSMART Cloud portal.

The Lux3 FL can image and analyze various applications, for example, monitoring cell viability, determining transfection efficiency, investigation of co-cultures, and analyzing cellular processes. Researchers working in drug discovery, tissue engineering, immunology, immunotherapy, and cancer research fields can benefit from the fluorescence live-cell imaging.

Yes, viability is one of the numerous applications that fluorescence live-cell imaging has to offer.


There are various fluorescent dyes on the market to analyze cell viability, and we can share our recommendations. Feel free to contact us for more information.

Many different fluorescent dyes can be used, as long as the fluorescent dye's excitation and emission spectra correspond with the fluorescent filters of the Lux3 FL (Green: Excitation: 452/45 nm, Emission: 512/23 nm. Red: Excitation: 561/14 nm, Emission: 630/90 nm). Some examples are RFP (red fluorescent protein), PI, and Cell Tracker Orange, for the red channel, and GFP (green fluorescent protein) and Cell Tracker green for the green channel.



It is essential to match the fluorescent dye with the specifications of the device. Next to this, it is important to ensure that the dye is not toxic for the cells since you want to use it on live cells.

Magnification is equal to a standard microscope with a 10X and 20X lens.

Yes, with the Lux3 FL, it's possible to set the LEDs' intensity for red and green fluorescent channels according to users' preferences.

Both fluorescence channels are included in the device. Researchers can make time-lapse videos with the brightfield, red and green channel imaging in parallel. Of course, it is also possible to choose just one of the fluorescent channels, depending on the experiment's goal and the researcher's preferences.

Images can be recorded at pre-defined intervals. At the start of a new experiment, you can specify the interval rate anywhere between 5 min and 12 hours.

The CytoSMART Lux3 FL allows monitoring of a wide range of different culture dishes and flasks, such as:


  • ​T-flasks: T-25 up to T-250
  • Single well, multi-well plates (6, 12, 24, 96 well)
  • microfluidic chips
  • Flat tubes
  • Petri dishes
  • Slides

The system requires a dedicated PC or laptop running on windows 10 or above and with a USB3 port and an active internet connection. If your lab doesn’t have the sufficient hardware, we can supply these for a fee as part of your order.

The CytoSMART Lux3 FL remote functionality runs on cloud-based software. In this cloud-environment, the images and videos are stored and can be accessed using user-specific login details. Next to unlimited data storage, automated image analysis can be performed in the CytoSMART Cloud portal.

The devices are easy to clean using lint-free wipes and EtOH (70%) or IPA. Do not use Acetone to clean the device. Please be aware that the device cannot be autoclaved. After sterilizing with EtOH or IPA the device can be used in a cleanroom.

This is not necessary. However, to get the best results, we advise covering the fluorescence microscope to minimize the amount of environmental light as much as possible. Turning off the lights in the room can also help to minimize the environmental light.

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