Being able to monitor cells over time, provides great insight in their dynamics and function. Video monitoring opens up novel and exciting avenues, to study cell health and viability, colony formation, migration and cellular responses to external factors.
To help researchers in life sciences to continue improving their understanding of cellular processes, CytoSMART has developed an automated bright-field microscope that visualizes complete culture vessels and operates from inside standard CO2-incubators. Perform kinetic assays that capture cellular behavior for days or even weeks at a time.
Continuous imaging provides real-time insight into cellular processes
Perform kinetic assays over the course of days or even weeks.
Pinpoint important events in the progression of the cell culture from experiment to experiment. Uncover attachment and detachment rates, elvaluate events like cell death and compare growth rates. Data collection using video monitoring allows users to capture changes within samples and compare the rate of change between samples.
Complete overview of sample heterogeneity
Achieved by automated full plate scanning and image stitching
Manual handling and cell seeding can cause a variable density distribution within culture vessels. Randomly selecting several areas of interest or tile scanning is common practice to overcome that issue, however this is either time-consuming to set-up or to post-process. The CytoSMART Omni automatically scans complete well surface areas and instantly stitches these images to give users a complete overview of cell coverage.
Visualizations from overview to cell level detail.
The CytoSMART Omni generates 6500 images at cell level detail; 10X magnification, 6 minutes for a full plate. These images are automatically stitched and provide a complete overview of the entire vessel that is used during assays and culturing.
- Label-free cellular assays
- Time-lapse videos
- Whole well surfaces
Would you like to test if the CytoSMART Omni is beneficial for your laboratory?
The CytoSMART Omni is ideal to perform kinetic assays to map cell proliferation, which we use as a tool to understand drug resistance in pancreatic cancer cell lines.
Dr. Martin Sprick | Senior Group Leader
Heidelberg Institute for Stem Cell Technology and Experimental Medicine
Understand Proliferation, Migration,
Colony counting & more.
Image analysis can be used to extract valuable information from time-lapse video's. Investigate and compare parameters like confluence, area infiltration and growth rate.
Perform analysis at desired culturing environment
The CytoSMART Omni is designed for your incubator
Live-cell imaging requires environmental control during the experiment. To ensure the proper environmental conditions, users can place the compact CytoSMART Omni inside any standard cell culture incubator. The smooth curvatures of the CytoSMART Omni platform ensures minimal airflow disturbance. This ensures that your samples stay in their desired, homogeneous environment throughout the entire experiment.
Ensure sample stability while imaging
Produce bright-field images without moving your samples
Acquiring images can by highly disturbing to living cells. Environmental shock, like sudden changes in the atmosphere and mechanical disturbance can negatively influence cell viability. The CytoSMART Omni rapidly captures bright field images at cell-level detail without disturbing the cells. Users can set the desired imaging parameters using their own computer and the CytoSMART Omni will follow that scanning regime. At no point after placing the cells on the sample stage does the culture need to be moved. Automated time-lapse imaging eliminates the need for any manual interference. Imaging using such a stable system prevents unwanted disturbances and reduces imaging artifacts
|Unit dimensions||396 x 345 x 171 mm (L x W x H)|
|Optics||Bright-field with digital phase contrast|
|Magnification||10x fixed objective|
|Camera||5 MP CMOS|
|Scan area||99 * 131 mm|
|Exported formats||JPG, XLSX & MP4|
|Well plate types||6 - 96 well plates|
|Culture flask types||petri dishes, T25 - T225, triple flasks and HYPERFlasks|
|Other labware||Anything transparent and lower than 55 mm|
|Operating environment||5-40 °C, 20-95% humidity|
|Support||Via email and live chat|
|Research use only. Not intended for diagnostic purposes|
Cell Viability Analysis
Cell Cytoxicity Assay to Analyze Drug Response
Frequently Asked Questions
Inverted bright field microscopy is used and images are enhanced using digital phase contrast. The camera moves below the sample stage. Samples are illuminated using LED lighting and a scanning motion, which facilitates sequential image generation. One complete scan takes 6 minutes in which 6500 images are generated. These are captured and stitched to form an image of a 99*131 mm surface area. The images are uploaded to the CytoSMART™ Cloud, there they can be analyzed using our image analysis algorithms.
You can specify the interval rate between 1 - 24 h or choose to perform a single scan.
The magnification is equivalent to 10X objective of a traditional bright field microscope
Confluence, scratch analysis and colony detection are currently part of the image analysis software package. Users always have the option to download the raw image data and perform their own analysis.
Yes, the CytoSMART Omni is designed to be used inside a cell culture incubator. Its hardware and electronics can operate at 5 - 40 °C and between 20 - 95% humidity.
No, our image analysis algorithms are optimized to be used in label-free assays, so you don’t have to add (toxic) dyes to your cells, providing a non-invasive analysis of your cells.
Yes, the device can only be used with a Windows-based computer with a USB 3.0 port (which can also be purchased at CytoSMART™). A WiFi or wired ethernet connection is necessary to be able to connect to the CytoSMART™ Cloud for data storage and analysis.
Any culture vessel that is lower than 55 mm (height of the light arc) can be scanned. However, the size of the scan is limited to 99*131 mm, which fits a complete T175 flask.
Some examples include 6-96 well plates, petri dishes, T25 - T225, triple flasks and HYPERFlasks.
Yes, after sterilizing with ethanol (70%) or IPA, the device can be used in a cleanroom. Do not use Acetone to clean the device, also the device cannot be autoclaved.
Cell Viability Analysis
Cell viability, growth and cytotoxicity studies can be performed using metabolic activity assays. The overall metabolic activity of the cell is indicated by the enzymatic cleavage of colorimetric or fluorescent substrates.
While these assays are relatively straightforward and cheap, they are dependent on culture conditions and intrinsic metabolic activity of the cell type that is being investigated. Furthermore, depletion of the metabolic substrate can lead to a plateau in the fluorescent signal, making assay output unreliable. To overcome these limitations cell viability could be determined optically using confluency measurements.
In the study described here the performance of confluency measurements to assess cell viability were compared to a metabolic activity assay: cell titer blue. Confluency was visualized using automated bright-field microscopy and subsequently analyzed using image analysis algorithms. Images were collected inside a CO2-incubator, keeping the culture at optimal conditions. For the cell titer blue assay resazurin was added to the medium and incubated for 3 hours. The fluorescent signal was normalized to the control to obtain the relative metabolic activity as a measure of cell viability. The comparison between the methods was performed for two pancreatic cancer cell lines, PACO7 and POCA43.
Cell Cytoxicity Assay to Analyze Drug Response
The effect of drugs and drug candidates on the viability of cells in culture can be determined using cell counting, live/dead assays and metabolic assays. However, these assays are often end-point measurements. Alternatively, cells can be monitored using bright-field microscopy, by creating time-lapse videos for a culture period of multiple days. To study the lasting effect of the drug candidate.
In this study the cytotoxic effect of Paclitaxel, a chemotherapy drug, was investigated for a range of concentrations. The effect on cell viability between drug concentrations was compared by analyzing confluency measurements obtained using automated live-cell imaging. The entire experiment was performed inside a CO2-incubator, ensuring optimal culturing conditions and cells were imaged every hour for a period of 3 days.
Cell migration is essential for physiological development and homeostasis, among other things. It is part of processes such as angiogenesis and wound healing. Conversely, cell migration in pathologies, including cancer can lead to worsening and progression of the diseased state.
To gain insight into collective cell migration a variety of assays have been developed. One of which is the wound healing assay, also known as the scratch assay. In this procedure a ‘wound’ is made in a confluent monolayer of cells, after which the gap closure is quantitatively monitored.
For this study a wound healing assay was performed to assess the effect of the drug paclitaxel on the migration of C6 rat glail tumor cells. Automated live-cell imaging was performed inside a CO2-incubator to ensure cells are kept at the desired conditions during the entire experiment. The experiment was performed in a 24 well plate, which was fully imaged every hour for 23 hours. The surface area and gap closure speed were compared for a concentration range of paclitaxel.