The Organoid Counting application of the Corning Cell Counter examines organoids of varying shape and size using bright-field image analysis.
Together with Corning®, CytoSMART Technologies has already introduced an automated cell counter for counting mammalian cells called the Corning® Cell Counter. Additionally, a new, powerful, and user-friendly software has been developed that enables organoid counting with the Corning® Cell Counter.
Manual organoid counting is a routine procedure for many research laboratories, but is time-consuming and user-dependent. To overcome these issues CytoSMART has introduced an organoid counting application that is:
- Accurate - powered by artificial intelligence, the Organoid counting software allows data gating and image selection for performing statistical analyses of acquired data
- Fast - the software can analyze a single image in less than three seconds* utilizing the CytoSMART™ Cloud (*at a 73 Mbps download and 20 Mbps upload speed)
- Low-cost - the counter is compatible with a standard, reusable glass hemocytometer. No consumables required
State-of-the-art, machine learning algorithm for rapid organoid detection
The CytoSMART Organoid Counting software is powered by an image analysis algorithm optimized for organoid detection. Users obtain information on the quantity and size of the organoids in a sample. This data is displayed in separate interfaces that provide a clear overview of the characteristics of the organoid population, including:
- Organoid size distribution
- Average sample concentration
- Individual images of the sample
NEW - Organoid morphology assessment
Detection of spherical and irregularly-shaped organoids
The shape of organoids is dependent on a variety of factors, including the physical features of cells that form organoids, cell proliferation rates, and cell differentiation capacity. The newly updated Organoid Counting algorithm is designed to recognize and quantify not only irregularly-shaped organoids, but also more structured, spherical organoids. In addition, the new algorithm is better at differentiating organoids from cellular debris, thus reducing the amount of false-positives/false-negatives.
Evaluation of organoid size and concentration
Accurate and reproducible organoid counts
Organoid size can be highly variable. If a large organoid covers the entire field of view, and a single image of that large organoid is used for the concentration estimation, then the smaller organoids that surround it, are neglected and organoid concentration of the sample is underestimated. Users can overcome this issue by taking multiple images of the sample at various positions within the counting chamber. Using the data obtained from multiple images, the accuracy of the organoid count drastically increases.
Accessible data anywhere, anytime
The Organoid Counting software instantly generates a report containing organoid number and size. The reports are then sent to the CytoSMART™ Cloud, which enables you to access the analyzed image data on your smartphone, tablet, or computer at any time.
Learn more about the Corning Cell Counter here
|Organoid size range||10 to 100/200 μm**|
|Measurement time||<3 secs*|
|Compatibility||Reusable and disposable counting chambers with 0.1 or 0.2 mm height|
|Sample volume||10 μl***|
|Field of view||1.5 mm x 1.5 mm|
|Image resolution||1536 x 1536|
|* Measured using a 73 Mbps download speed and a 20 Mbps upload speed|
|** Size limits depend on dimensions of counting chamber, user can set limits in application|
|*** For Neubauer counting chamber with 0.1 mm height|