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Live-cell imaging: illuminating cells in real time

Live-cell imaging is a technique, whereby the cells are imaged when they are alive, typically in the growth medium. In recent years, live-cell imaging has become a requisite analytical tool for addressing important questions in several areas of biological and biomedical sciences including cancer research, neuroscience, cell biology, developmental biology, and pharmacology.

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RESOURCES

Manual vs automated cell counting, is there a great divide?

Automated cell counters have become very popular research tools, however, many labs are still hesitating to invest in an automated cell counter. This article summarizes the key points that need to be considered when deciding between an automated or manual setup.

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Live-cell imaging in the field of cell therapy

Cell therapy has broad applications that range from treating cancer, diabetes, cardiovascular and neurogenerative conditions to tissue regeneration and replacement. CytoSMART offers an excellent range of live-cell imaging systems that can be used to research and manufacture cell therapies. The applications and examples of these live-cell imaging systems will be discussed below.

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PRESS RELEASES

After a successful winter initiative, the CytoSMART Research Grant project resumes again

6 September 2021, Eindhoven, The Netherlands - Today, CytoSMART Technologies has opened the application for the CytoSMART Research Grant Autumn 2021. The CytoSMART Research Grant is an initiative to help motivated students and researchers improve their project's quality. The grant offers one of the CytoSMART live-cell imaging devices that can be used for research purposes for a specified period. The CytoSMART automated, incubator-friendly microscopes are suitable for monitoring cell cultures in any research topic that could benefit from live-cell imaging. The application period starts on September 6 and will last until October 15, 2021.

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PRESS RELEASES

CytoSMART introduces new, label-free live-cell microscopy solutions with high image quality

10 August 2021, Eindhoven, The Netherlands - CytoSMART Technologies today announced the launch of the new brightfield live-cell imaging system – the CytoSMART Lux3 BR. It is a small brightfield microscope, equipped with a high-quality 6.4 MP CMOS camera. The new live-cell imager is designed to work inside a standard cell culture incubator as all other CytoSMART microscopes, without disturbing temperature, airflow, and optimum culture conditions for the cells. This allows researchers to perform long-term live-cell imaging experiments, ensuring optimal cell growth and health. Very detailed brightfield images can be captured using the new device. In both x- and y-direction, 2072 pixels combined with 1.45 mm field of view provide a resolution of 0.7 µm/pixel. Even at the commonly required image resolution of 300 dpi for printed (scientific) publications, these images can fill the entire page width if desired, without compromising the image quality.

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E-BOOKS

E-book: The Clonogenic Assay

Today the clonogenic assay, also known as the colony formation assay, is widely used in the field of cancer research as the formation of clones is interpreted as a trait of cancer cells with tumor-initiating capabilities. In this e-book, we discuss the evolution of clonogenic assay, its examples, different ways to perform the clonogenic assay, most common FAQs, and more.

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5 factors to consider when choosing a sustainable live-cell imaging system

This article highlights considerations for more sustainable live-cell imaging. Live-cell imaging enables researchers to determine not only whether, but also when and how certain cellular events occur in culture. Therefore, this technique is increasingly applied in cell research. The environmental footprint of live-cell imaging can be large, although more sustainable alternatives are also available within this experimental procedure. When looking for a live-cell imaging system, the following 5 factors can already increase sustainability with minimal effort: light source, connected computer, data storage, remote monitoring and analysis, and system complexity.

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CYTOSMART ACADEMY

Life Scientists Horror Stories (Part 1)

In this blog post, life scientists share their true lab horror stories and lessons they learned the hard way. Do you have your own lab horror story? Feel free to share it with us!

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To automate, or not to automate, that’s the question

Laboratory automation is becoming a new reality in scientific research, due to the rapid technological advancement and the numerous benefits offered by this strategy. Here, we explain relevant commonly used terms in the field of laboratory automation, provide factors to be considered before automating a laboratory, summarize requirements for a successfully automated laboratory, and identify typical laboratories and sectors that fulfil the requirements and, therefore, could be easily and successfully automated.

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Visualizing astroglial motility in a mouse model of Alzheimer's disease using live-cell imaging [Interview with Dr. Lusine Danielyan]

A recent study by Drews et al. (2021) focuses on examining broad neuroprotective qualities of losartan. The CytoSMART Lux3 FL fluorescence live-cell imager was used to monitor the motility of Aβ-affected astrocytes. To learn more about the use of the CytoSMART devices in life sciences, we asked Dr. Lusine Danielyan a few questions about her experience with using the Lux3 FL for live-cell imaging.

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