Introduction
Live-cell imaging enables researchers to determine not only whether, but also when and how certain cellular events occur in culture. When compared to brightfield-only imaging, fluorescence live-cell imaging multiplies the number of read- outs– and consequently the obtained information from one experiment – with the number of fluorescence channels. In cellular co-cultures, fluorescence imaging facilitates distinction of the cell types, when cell types are assigned specific fluorescent labels. Quantitative and qualitative read-outs per cell type are then based on the respective fluorescent labels [1].
