Authors: Harlaar N, Dekker SO, Zhang J, Snabel RR, Veldkamp MW, Verkerk AO, Fabres CC, Schwach V, Lerink LJS, Rivaud MR, Mulder AA, Corver WE, Goumans MJTH, Dobrev D, Klautz RJM, Schalij MJ, Veenstra GJC, Passier R, Brakel TJV, Pijnappels DA, Vries AAF.
Nature Biomedical Engineering 6, 2022
In this study, the authors show that fully functional atrial myocytes can be produced and expanded by means of a conditional cell-immortalization approach that relies on lentiviral vectors and the expression of a viral oncogene in human fetal atrial myocytes. The Exact (Corning Cell Counter) was used to assess the proliferation rates of human immortalized atrial myocytes (hiAMs). All in all, this study provides proof-of-concept of a new method to generate considerable numbers of human cells for the creation of representative human in vitro models.
