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Wound healing assays: Cell outgrowth assays
Cell outgrowth assays, also referred to as nest assays or radial migration assays, can be used to investigate collective cell migration in fields such as oncology and drug discovery.
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Wound healing assays: Single-cell migration assays
Cell migration is involved in a multitude of critical physiological and pathological processes. Cell motility can be divided into collective and single-cell migration. Single-cell migration is used by cells to move towards and between tissues, and it can be split into amoeboid and mesenchymal migration. A variety of in vitro assays have been developed to study single-cell migration.
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Cell viability assays: why, what and how
Cell viability is a measure of the proportion of live cells within a population. The measurement of cell viability plays an essential role in all forms of cell culture.
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Cell proliferation assays supported by live cell imaging
Live-cell imaging supports standard end-point biological assays assessing proliferation, metabolic activity and viability, immunological responses, and DNA damage by enabling monitoring of cellular confluence, proliferation, and viability in real-time.
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Seeing is believing: Label-free time-lapse microscopy for quantifying cell proliferation
Cell proliferation assays are used in a wide variety of contexts. For example, proliferation can be used to gauge the health of new transgenic cell lines or to evaluate the success of tissue regeneration experiments. Quantitative cell proliferation assays are also essential for evaluating the dose-response of new pharmaceutical agents, such as in the context of anti-cancer drug development.
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Optical Microscopy vs. Digital Microscopy: For cell cultures studies
A variation of the optical microscope is the digital microscope. These microscopes use a digital camera instead of eyepieces. Images are seen in real-time on a computer screen. The invention of the USB port in the 1990s has made available a wide variety of USB digital microscopes to suit every need.
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Live-cell imaging: A journey from hand-drawn sketches to microcinematography
Live-cell imaging is the study of living cells using time-lapse microscopy. It is used by scientists to obtain a better understanding of biological function through the study of cellular dynamics. Live-cell imaging has offered countless insights into biological marvels. It is the study of living cell/s with time-lapse microscopy. It helped scientists to achieve a better understanding of biological function through the study of cellular dynamics.
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Remote monitoring of iPSC-derived neural crest cells
Neuronal cells used in in vitro research are often derived via directed differentiation of human-induced pluripotent stem cells (iPSCs). In order to make sure the iPSCs differentiate into neuronal cells, it is very important to keep a close eye on your cells during this costly 30-day process. CytoSMART’s live-cell imaging microscopes and Anatomic's 7-day neural differentiation method can speed up this process.
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Wound healing assays: discussing subtypes of cell removal assays
Cell removal assays are generally a low-tech solution to study migration and wound healing. Here we discuss and compare the stratch assay, stamp wounding, PDMS barrier assay, laser-based wounding, electrical wounding, chemical wounding and vacuum-based wounding.
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Hypoxic conditions: The low down on the importance of hypoxia in stem cell culture
As with other in vivo tissues, stem cell niches are an hypoxic environment and exhibit oxygen levels that are notably lower than the ambient environment. Indeed, in most tissues, physiological normoxia ranges from <1-9% oxygen, and stem cell niches are no different. Hypoxia chambers can help to emulate these environments.
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