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Fluorescence live cell imaging applications
The emergence of fluorescent tools has started a new era in biological research and enabled us to see and study cellular processes and dynamics that were not possible before.
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The emergence of fluorescent tools has started a new era in biological research and enabled us to see and study cellular processes and dynamics that were not possible before.
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Various applications, such as monitoring cell viability, determining transfection efficiency, investigating co-cultures, and analyzing cellular processes, can benefit from the fluorescent labels and their detailed kinetic visualization.
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With advancements in instrumentation, technologies, and the overcoming of a number of technical challenges, live-cell imaging has enabled researchers to study cellular dynamics with enhanced spatial and temporal resolution.
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An infographic about how researchers can optimize their research budget and save unnecessary costs using live-cell imaging.
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CytoSMART infographic about fluorescence live-cell imaging, main challenges, and fluorescent experiments using live-cell imaging.
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Cellular heterogeneity essentially observes all dimensions of a single cell. Heterogeneity tracking helps us to measure the functionality of each cell. An example of its common use is to track cancer patients’ cell or tumour growth or decrease over time.
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Cell exclusion zone assays differ fundamentally from wounding assays; instead of removing part of the culture, they provide an artificial barrier to the culturing area.
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Cell outgrowth assays, also referred to as nest assays or radial migration assays, can be used to investigate collective cell migration in fields such as oncology and drug discovery.
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Cell migration is involved in a multitude of critical physiological and pathological processes. Cell motility can be divided into collective and single-cell migration. Single-cell migration is used by cells to move towards and between tissues, and it can be split into amoeboid and mesenchymal migration. A variety of in vitro assays have been developed to study single-cell migration.
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Cell viability is a measure of the proportion of live cells within a population. The measurement of cell viability plays an essential role in all forms of cell culture.
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