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In this article we will discuss the clinical use, isolation and culturing specifically for hUC-MSCs. It will become clear that maintain a strict culturing protocol, sticking to specific seeding densities, media and observing confluence is essential to maintain stemness of the MSCs.
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A look into their definition, culturing methods, and therapeutic potential
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A clonogenic assay, also known as a colony formation assay, is an in vitro cell survival assay. It assesses the ability of single cells to survive and reproduce to form colonies. This blog highlights: Clonogenic assay applications. How to perform a clonogenic assay with important considerations to make along the way. Quantifying your colonies and plotting a survival curve. Using label-free microscopy and confluence detection as an alternative, time and cost effective clonogenic assay protocol
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Despite all the advancements in iPSC technologies, culturing iPSCs still remains a challenging and complicated process. There are now more options than ever for reprogramming methods, growth medias, cell attachment substrates, dissociation reagents, and passaging techniques. Ultimately there is no one way to grow iPSCs.
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This blog outlines the various therapeutic applications of bone marrow-derived MSCs (BM-MSCs), their isolation and culturing methods. Also described are quality control measures, including visual inspection criteria, that are useful for ensuring stemness i.e. self-renewal and multi-lineage differentiation potential.
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Irreproducible results? Don’t blame fraud, blame confluency. How inconsistent culturing conditions brings about inconsistent results in cell metabolism studies.
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A central challenge in live-cell imaging maintaining physiological cell behavior. Specimens removed from their native source may exhibit morphological or behavioral changes. Cell health and behavior can be controlled during imaging by keeping cells in a culture system that closely resembles their physiological growth environment.
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We look at general benefits, equipment requirements and ask what metrics we want to extract. Cell populations or (sub)cellular information?
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compound efficacy assays that study cell death by analysis of growth curves
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Study live cells using non-invasive & non-toxic methods
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