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Spheroids have become an exciting cell culture model, as they better mimic in vivo tissue properties and exhibit a superior capacity for differentiation compared to 2D culture systems. These properties make them well suited for use in drug development and tissue engineering studies, as well as for the study of fundamental biological principles.
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In this article we will go into how the proliferative rate and confluency of cells (1) affect baseline gene expression levels, and consequently, the biological interpretation of those data, and (2) influence the efficiency of gene reprogramming and the quality of the resultant products.
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CPE is a very basic approach to understand how a virus infects a cell, but that does not mean it is just used in basic scientific research. Measuring CPEs can also be a very useful readout for pharmaceutical companies and diagnostic laboratories.
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Establishment of protocols for the extraction or differentiation of neural stem cells (NSCs) and their expansion in vitro was a great advancement in neurobiology. NSCs grown in culture provide a powerful tool and an accessible model to investigate human neurodevelopment and cell biology. This article discusses neural stem cell sources, isolation, culture methods and differentiation potential.
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In this article we will discuss the clinical use, isolation and culturing specifically for hUC-MSCs. It will become clear that maintain a strict culturing protocol, sticking to specific seeding densities, media and observing confluence is essential to maintain stemness of the MSCs.
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A look into their definition, culturing methods, and therapeutic potential
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A clonogenic assay, also known as a colony formation assay, is an in vitro cell survival assay. It assesses the ability of single cells to survive and reproduce to form colonies. This blog highlights: Clonogenic assay applications. How to perform a clonogenic assay with important considerations to make along the way. Quantifying your colonies and plotting a survival curve. Using label-free microscopy and confluence detection as an alternative, time and cost effective clonogenic assay protocol
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Despite all the advancements in iPSC technologies, culturing iPSCs still remains a challenging and complicated process. There are now more options than ever for reprogramming methods, growth medias, cell attachment substrates, dissociation reagents, and passaging techniques. Ultimately there is no one way to grow iPSCs.
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This blog outlines the various therapeutic applications of bone marrow-derived MSCs (BM-MSCs), their isolation and culturing methods. Also described are quality control measures, including visual inspection criteria, that are useful for ensuring stemness i.e. self-renewal and multi-lineage differentiation potential.
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Irreproducible results? Don’t blame fraud, blame confluency. How inconsistent culturing conditions brings about inconsistent results in cell metabolism studies.
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