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Organoids (also called “mini-organs”) are three-dimensional (3D) in vitro cell clusters that self-organize and differentiate into functional cell types, recreating aspects of in vivo organ biology. The organoids’ capacity to resemble – to a certain degree – complex organs make them an ideal model for a multitude of applications ranging from studying the fundamentals of developmental biology to investigating the molecular mechanisms of infectious diseases and cancer. The CytoSMART systems combine affordability, high quality, and user convenience, making them an excellent tool for studying more complex cell models, such as organoids.


With automated in vitro 3D cell culture model analysis:


Track organoid development using label-free analysis in real time and in physiologically relevant conditions

Organoids represent an exciting avenue for disease research and discovery, bridging the gap between traditional 2D cell cultures and animal models. Identify, track, and analyze large numbers of organoids across an array of culture vessels with advanced machine-learning algorithms for faster, more accurate results.

Here, organoids embedded in Matrigel were tracked using the CytoSMART Omni to assess their growth and morphology and optimize organoid culture method.

Assess the number, size, eccentricity, and population distribution of organoids in a sample

Organoids come in many shapes and sizes due to a variety of factors, including cell type, disease phenotype, and even culture conditions. The Organoid Analysis Module for the CytoSMART Omni is able to detect and quantify a diverse range of organoid phenotypes.

Count organoids and other 3D cell cultures

Organoids are three-dimensional (3D) in vitro cell culture models that have been used to study infectious diseases, genetic disorders, and cancers. Accurately measuring organoid concentration is critical for developing and improving treatments and therapies. The number, size, and population distribution of organoids in a sample can be assessed using the CytoSMART Exact platform.

The data displayed in separate interfaces provides a clear overview of the characteristics of the analyzed organoid population.