The CytoSMART Exact FL is an automated, dual fluorescence cell counter with an expanded field of view and unmatched resolution and magnification power. Using an advanced CMOS-based optical system and an image analysis software powered by Artificial Intelligence (AI), the CytoSMART Exact FL calculates the exact number of cells in a sample and provides reliable assessment of key cellular parameters, including cell viability and transfection efficiency. The increased magnification and high resolution offer an unprecedented level of detail, allowing to distinguish between whole cells and cell debris and count even the smallest of mammalian cells (CAR-T cells, stem cells, PBMCs or splenocytes).
- State-of-the-art optics – a 6.4 MP CMOS camera combined with 10× magnification allow to accurately distinguish individual cells in clumps and count cells down to 4 µm in diameter
- AI-driven image analysis – cell counting is performed by highly trained pattern recognition software, minimizing user-to-user variability
- Large field of view & multi-count – by taking multiple images of the same sample at various slide positions, a larger portion of the available counting volume can be analyzed. Increased counting volume provides significantly lower coefficient of variation values, as compared to traditional image based cell counters
- Add-ons – the CytoSMART Exact FL is the first cell counter with an expandable application range, including an additional module for counting organoids
- Fastest autofocus - the Exact FL has the fastest autofocus system on the market that minimizes user inputs and variability
The CytoSMART Exact FL is very user friendly and makes time consuming work faster and easier.
"The CytoSMART Exact FL is very user friendly and makes time consuming work faster and easier. Taking the microscope images was simple with the straightforward software which analyses the images and determines the cell counts and viability for you. The data can be exported in different formats as well as being automatically uploaded to a cloud service. The technical support for the machine was great and I couldn't have asked for better customer service."
Count even the smallest of mammalian cells
Mammalian cells come in all shapes and sizes. The CytoSMART Exact FL has far better optics compared to conventional systems. Using the 6.4 MP CMOS camera combined with 10× magnification, the CytoSMART Exact FL can visualize and count cells down to 4 µm in diameter. With increased field of view and multi-count feature, it is also possible to take multiple snapshots of the same sample at various slide positions, which increases the accuracy and reproducibility of a cell count.
Dual fluorescence cell counting
Expand the possibilities of automated cell counting
Primary cell-based assays can significantly benefit from fluorescence cell counting due to the number of issues associated with detecting primary cells using colorimetric dyes (e.g. Trypan Blue). The CytoSMART Exact FL, fitted with red and green fluorescent channels, in combination with appropriate fluorescent dyes, can evaluate viability and concentration of primary cell samples with maximum accuracy and reproducibility.
Innovative organoid counting software
Organoids are stem cell-derived 3D culture systems that have been used to study genetic disorders, infectious diseases, and various types of cancer. To ensure consistency and reproducibility across multiple organoid-based experiments, it is critical to use a fixed concentration of organoids of similar size. Using the Organoid Counting add-on module, the CytoSMART Exact FL can analyze brightfield images to determine a precise number and size of organoids in a sample.
Freedom to choose between reusable or disposable counting slides
Use slides that work best for you
The CytoSMART Exact FL is compatible with a range of plastic and glass microscope slides, including the CytoSMART disposable and reusable counting slides. These slides can be used for counting both, individual cells and organoids.
Automated software updates & secure cloud data storage
All CytoSMART devices are connected to the CytoSMART cloud environment. This means that the data generated by the Exact FL fluorescence cell counter is processed, analysed and stored in one accessible and secure place, protected by a multi-layered cyber security system. Novel software updates are also introduced via the CytoSMART Cloud. With only a few clicks our customers can download and install these add-ons to make their user experience even more seamless. Importantly, the added features will not affect consistency and reproducibility of acquired results.
|Counting range (concentration/mL)||(5.0 × 10^4) – (1.0 × 10^7)|
|Size range - mammalian cells||4 – 70 µm|
|Size range - organoids||10 µm to 100/200 µm*|
|Loading volume (1 chamber)||10 µL|
|Field of view||1 count: 2.1 mm^2|
|8 counts (multi-count): 16.8 mm^2|
|Option for multi-count||Yes|
|Channels||Brightfield, green and red fluorescent channels|
|Fluorescence filters||Green – excitation: 452/45 nm; emission: 512/23 nm|
|Red – excitation: 561/14 nm; emission: 630/90 nm|
|Magnification||10× (digital zoom 20×)|
|Image resolution||2072 × 2072 pixels|
|Camera||6.4 MP CMOS|
|Data formats||JPG, CSV, PDF|
|Data storage||The CytoSMART Cloud|
|Computer requirements||Windows 10, 2.4 GHz i5 processor, 4 GB RAM memory, USB 3.0, internet|
|Measurement time**||3 sec|
|Counting chamber||Reusable (glass) or disposable (plastic) slides|
|Dimensions (L × W × H)||16.6 cm × 14 cm × 13.5 cm|
|Warranty||1-year and 3-year***|
|Extra features||Live gating|
|Organoid counting module|
|* size limit depends on the counting chamber dimensions|
|** at 73 Mbps download and 20 Mbps upload speed|
|*** if a purchase order is made before July 31, 2021|
|Research use only. Not intended for diagnostic purposes|
Fluorescence cell counting Application Guide PDF
Fluorescence-cell-counting-Application-Guide-PDF ( PDF - 281 KB )
Exact FL Product Features 1 pager PDF
Exact-FL-Product-Features-1-pager-PDF ( PDF - 160 KB )
Exact FL Product Features 2 pager PDF
Exact-FL-Product-Features-2-pager-PDF ( PDF - 984 KB )
Exact FL Brochure PDF
Exact-FL-Brochure-PDF ( PDF - 3 MB )
Exact FL Compatible Fluorescent Proteins PDF
Exact-FL-Compatible-Fluorescent-Proteins-PDF ( PDF - 173 KB )
Exact FL Compatible Staining PDF
Exact-FL-Compatible-Staining-PDF ( PDF - 105 KB )
Frequently Asked Questions
Many different fluorescent dyes can be used, as long as the fluorescent dye’s excitation and emission spectra correspond with the fluorescent filters of the Exact FL (green – excitation: 452/45 nm, emission: 512/23 nm; red – excitation: 561/14 nm, emission: 630/90 nm). Some examples are propidium iodide (PI) and red fluorescent protein (RFP) for the red channel, and acridine orange (AO), calcein-AM, and green fluorescent protein (GFP) for the green channel.
No, the Exact FL is not suitable for counting non-mammalian cells.
Yes, cell viability can be assessed either using green and red fluorescent channels (AO/PI) or a brightfield channel (Trypan Blue). When the green and red fluorescent channels are deselected, the Trypan Blue option will appear automatically.
It is possible to distinguish white blood cells from non-nucleated red blood cells and platelets by using a nuclear dye (e.g. acridine orange).
There is no specialized algorithm that can distinguish between different subpopulations of white blood cells. However, it is possible to distinguish these various cell types based on their size and density, using the size gating slider.
No, there is no specific algorithm available. However, it is possible to study mitosis phases either using a cell cycle-specific dye (e.g. FUCCI staining) or a nuclear dye. The nuclear dye together with the intensity slider can allow to measure cells' DNA content, thus differentiating between the cell cycle phases.
Yes, as long as the cell counting chamber is transparent and has a depth of 0.1 mm. It does not matter if the cell counting chamber is made of glass, plastic, has a counting grid or if there are multiple counting chambers.
No, this is not necessary. The cell counting algorithm is specifically designed for counting chambers with a depth of 0.1 mm. When using a counting chamber with a different depth, you have to adjust the dilution factor to get accurate results.
The device requires a dedicated desktop or laptop running on Windows 10 or above, with a USB3 port and an active internet connection. A WiFi or Ethernet connection is necessary for connecting to the CytoSMART Cloud for image analysis and storage.
No calibration of the device is required.
To get the best results, we advise covering the stage of the device with the provided adapter plate to minimize the amount of environmental light.
Yes, it is possible to set the intensity of the LED for red and green fluorescent channels according to users’ preferences.
Yes, the intensity values are the average intensity of that specific count.
The device is easy to clean using lint-free wipes and ethanol (70%) or isopropyl alcohol (IPA). Do not use acetone to clean the device, also the device cannot be autoclaved.